primary antibodies aqp1 proteintech Search Results


90
Cell Signaling Technology Inc kdr antibody
Kdr Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech aqp1
Aqp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology skim milk powder
Skim Milk Powder, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech antibodies against aqp1
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Antibodies Against Aqp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibodies against aqp1/product/Proteintech
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Proteintech aqp1 marker
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Aqp1 Marker, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Affinity Biosciences anti-aqp-1 af5231
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Anti Aqp 1 Af5231, supplied by Affinity Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Millipore aqp1 antibody
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Aqp1 Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Proteintech rabbit anti aqp1
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Rabbit Anti Aqp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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95
Proteintech 1 ig
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
1 Ig, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
Cell Signaling Technology Inc p-iκkα/β
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
P Iκkα/β, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Proteintech anti aqp1
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Anti Aqp1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti aqp1/product/Proteintech
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93
Proteintech antibodies against aquaporin 1
Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with <t>Aqp1</t> and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Antibodies Against Aquaporin 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with Aqp1 and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse

doi: 10.3389/fcell.2023.1171637

Figure Lengend Snippet: Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with Aqp1 and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.

Article Snippet: After blocking with 5% bovine serum albumin in PBST at room temperature for 1 h, the slides were immunostained with primary antibodies against Aqp1 (20333-1-AP, Proteintech, 1:100), NKCC2 (18970-1-AP, Proteintech, 1:100), megalin (sc-515772, Santa Cruz, 1:100), Calbindin (66,394-1, Proteintech, 1:50), Aqp2 (ab62628, Abcam, 1:100), podocalyxin (AF1556, R&D Systems, 1:100), and GFP (50,430-2, Proteintech, 1:100) at 4°C overnight.

Techniques: Expressing, Activation Assay, Activity Assay, Staining

Efficient recombination occurred in renal proximal tubular cells of AMN CreERT2 ; Rosa26-LTL-tdTomato mice after tamoxifen administration. (A) The induced Cre recombination efficiency in proximal tubule epithelial cells of AMN CreERT2 mice with different dosages of tamoxifen. In the control group, no leak Cre activity was observed in the whole kidney without tamoxifen induction. The total number of tdTomato-positive cells significantly increased with the rise in tamoxifen dosage. (B) Induction efficiency in different tamoxifen dose groups. The ratio of Aqp1 + /tdTomato + cells to Aqp1 + cells was measured in five randomly selected images per kidney ( n = 4). Three asterisks signify highly significant differences between different groups. (** p < 0.001). (C) Cell specificity of CreERT2 activity in the renal proximal tubular epithelial cells. The ratio of Aqp1 + /tdTomato + cells to Aqp1 - /tdTomato + cells in five randomly selected images were measured per kidney ( n = 4). Scale bar = 50 μm.

Journal: Frontiers in Cell and Developmental Biology

Article Title: Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse

doi: 10.3389/fcell.2023.1171637

Figure Lengend Snippet: Efficient recombination occurred in renal proximal tubular cells of AMN CreERT2 ; Rosa26-LTL-tdTomato mice after tamoxifen administration. (A) The induced Cre recombination efficiency in proximal tubule epithelial cells of AMN CreERT2 mice with different dosages of tamoxifen. In the control group, no leak Cre activity was observed in the whole kidney without tamoxifen induction. The total number of tdTomato-positive cells significantly increased with the rise in tamoxifen dosage. (B) Induction efficiency in different tamoxifen dose groups. The ratio of Aqp1 + /tdTomato + cells to Aqp1 + cells was measured in five randomly selected images per kidney ( n = 4). Three asterisks signify highly significant differences between different groups. (** p < 0.001). (C) Cell specificity of CreERT2 activity in the renal proximal tubular epithelial cells. The ratio of Aqp1 + /tdTomato + cells to Aqp1 - /tdTomato + cells in five randomly selected images were measured per kidney ( n = 4). Scale bar = 50 μm.

Article Snippet: After blocking with 5% bovine serum albumin in PBST at room temperature for 1 h, the slides were immunostained with primary antibodies against Aqp1 (20333-1-AP, Proteintech, 1:100), NKCC2 (18970-1-AP, Proteintech, 1:100), megalin (sc-515772, Santa Cruz, 1:100), Calbindin (66,394-1, Proteintech, 1:50), Aqp2 (ab62628, Abcam, 1:100), podocalyxin (AF1556, R&D Systems, 1:100), and GFP (50,430-2, Proteintech, 1:100) at 4°C overnight.

Techniques: Control, Activity Assay