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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse
doi: 10.3389/fcell.2023.1171637
Figure Lengend Snippet: Renal proximal tubular epithelial cell-specific expression and activation of the Cre recombinase in AMN CreERT2 mice. (A) Representative images showing the co-localization of GFP (green) and megalin (red) in renal proximal tubules. Rectangles depicted in the upper column were shown at a higher magnification in the lower column. (B) Diagram illustrating the generation of AMN CreERT2 ; Rosa26-LSL-tdTomato mice. (C) Expression of the tdTomato reporter gene in various tissues. The Cre activity (tdTomato + ) was only detected in the small intestine and kidney of the AMN CreERT2 ; Rosa26-tdTomato mice following tamoxifen induction. (D–I) The Cre activity was detected in renal proximal tubules in the cortex and medulla, which was co-stained with Aqp1 and LTL (D–E) . No Cre activity was detected in the thick ascending limb of the loop of Henle stained with NKCC2 (F) , distal tubules stained with calbindin (G) , collecting tubules stained with Aqp2 (H) , and the glomerulus stained with podocalyxin (I) . Scale bar = 100 μm (C, D) and 50 μm (A, E, F, G, H, I) . The high dose of tamoxifen was used in this set of experiments.
Article Snippet: After blocking with 5% bovine serum albumin in PBST at room temperature for 1 h, the slides were immunostained with primary
Techniques: Expressing, Activation Assay, Activity Assay, Staining
Journal: Frontiers in Cell and Developmental Biology
Article Title: Generation and characterization of an inducible renal proximal tubule-specific CreERT2 mouse
doi: 10.3389/fcell.2023.1171637
Figure Lengend Snippet: Efficient recombination occurred in renal proximal tubular cells of AMN CreERT2 ; Rosa26-LTL-tdTomato mice after tamoxifen administration. (A) The induced Cre recombination efficiency in proximal tubule epithelial cells of AMN CreERT2 mice with different dosages of tamoxifen. In the control group, no leak Cre activity was observed in the whole kidney without tamoxifen induction. The total number of tdTomato-positive cells significantly increased with the rise in tamoxifen dosage. (B) Induction efficiency in different tamoxifen dose groups. The ratio of Aqp1 + /tdTomato + cells to Aqp1 + cells was measured in five randomly selected images per kidney ( n = 4). Three asterisks signify highly significant differences between different groups. (** p < 0.001). (C) Cell specificity of CreERT2 activity in the renal proximal tubular epithelial cells. The ratio of Aqp1 + /tdTomato + cells to Aqp1 - /tdTomato + cells in five randomly selected images were measured per kidney ( n = 4). Scale bar = 50 μm.
Article Snippet: After blocking with 5% bovine serum albumin in PBST at room temperature for 1 h, the slides were immunostained with primary
Techniques: Control, Activity Assay